Out-of-equilibrium active membranes: incorporation of bacteriorhodopsin in floating lipid bilayer
Transport process through the membrane involves specific membrane proteins, which use metabolic energy as ATP hydrolysis or photochemical reaction to process conformational changes. This protein activity breaks the fluctuation-dissipation theorem leading to out-of-equilibrium fluctuations. These active fluctuations have been widely described theoretically but less is known on the experimental point of view. In the last 15 years, we have developed a new model system (fluid floating bilayer) and original off-specular reflectivity experiments allowing us to study the fluctuations of a single floating bilayer near a substrate as well as membrane-membrane interactions. It opens a wide range of perspectives to achieve a better understanding of active membranes properties which is the purpose of a PhD financed by ILL, in collaboration between ILL. In a recent experiment (9-13-580), we have obtained strong evidences of proteins (bacterio-rhodopsin) in supported DMPC bilayer. The next challenging step is to insert the proteins in floating bilayer and to investigate fluctuation enhancement by protein activation. This is the purpose of this proposal.
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charitat; DAILLANT Jean; FRAGNETO Giovanna; GERELLI YURI and MUKHINA Tetiana. (2017). Out-of-equilibrium active membranes: incorporation of bacteriorhodopsin in floating lipid bilayer. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-689
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