Understanding the interfacial behaviour of bile salts to better engineer lipid emulsions: neutron reflectivity measurements
The current project aims at elucidating how bile salts (BS) structure influences their interfacial behaviour and their impact on lipid digestion. Although the characterisation of BS interfacial properties is beginning to garner interest, the structure-function relationships governing their interfacial behaviour remain unknown. Neutron reflectivity (NR) is the only technique which can provide a detailed molecular picture of the BS/lipids interface, over time and at equilibrium. After studying the interfacial interactions of two distinct BS, sodium taurocholate and sodium taurodeoxycholate, with a 1,2-dipalmitoyl-sn-glycero-3-phosphocholine film, at the air/water interface, using the Langmuir trough and NR techniques, we would like to move onto the more physiologically relevant oil/water interface reflecting the actual fat droplet/water interface, using the spin-freeze-thaw method to trap a thin, uniform oil layer between a hydrophobically modified silicon substrate and an aqueous subphase. NR measurements will be employed to obtain information on the organisation of BS at the oil/water interface but also to monitor the interfacial changes caused by the addition of BS and enzymes.
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Dreiss; GERELLI YURI; GRILLO Isabelle; Richard D. Harvey; PABOIS Olivia; WILDE Pete and ZARBAKHSH Ali. (2018). Understanding the interfacial behaviour of bile salts to better engineer lipid emulsions: neutron reflectivity measurements. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-741
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