The melting of fiber DNA submerged in D-ethanol/D2O mixtures.
We are focused on finishing an ongoing project about studying the melting transition (or thermal denaturation) of DNA fibers submerged in different solutions. Namely solutions of polyethylene glycol in D2O and mixtures of D-ethanol and D2O. The peak that appears in the scattering pattern of this samples at Q=1.87 reciprocal Angstroms is related with the coherent scattering from closed base pairs and can be used to follow the evolution of the average size of the closed domainds during the transition. This average size is related to the coherence lenght of the distribution of closed basepairs which is a fundamental parameter for characterizing the transition. The part of the project focused on polymer solutions is completed and a publication is under preparation. We request now beam time in order to collect important missing data in the D-ethanol/D2O samples. These D-ethanol samples have showed a higher signal-to-noise ratio in comparison with the samples with long-chain polymers. The data suggest the ethanol mixtures affects the structure of the fibers differently from the polymer solutions.
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Adrian Gonzalez; S. Cuesta-Lopez; MOSSOU Estelle; PEYRARD Michel; THEODORAKOPOULOS Nikos and WILDES Andrew. (2018). The melting of fiber DNA submerged in D-ethanol/D2O mixtures.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-751
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