Incorporation and activation of transmembrane protein Archaerhodopsin-3 into floating phospholipid bilayers
Biological membranes consist of a complex mixture of various kinds of lipids, proteins and sugars. They undergo continuous interaction with the cytoskeleton matrix, other cells and different macromolecules and organelles present in the intracellular or extracellular cell interior.The plasma membrane is constantly subjected to the activity of its constituent components (pumping or channel activity of the proteins, isomerization processes, etc.), which changes its energy spectrum. On the contrary, pure lipid membranes exhibit only equilibrium thermal fluctuation due to the lipids' Brownian motion, so the addition of a non-equilibrium noise in physical models of lipid membranes is a necessary step towards a complete investigation of real biological membranes. In the project related to this proposal, we would like to address the question of the influence of the non-equilibrium fluctuations caused by the continuous protein activity on the structure of the phospholipid membrane. By means of NR we plan to demonstrate and fully characterize the insertion of the transmembrane protein AR3 into a floating lipid bilayer and to study the induced structural changes caused by AR3 activation.
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MUKHINA Tetiana; charitat; FRAGNETO Giovanna and GERELLI YURI. (2018). Incorporation and activation of transmembrane protein Archaerhodopsin-3 into floating phospholipid bilayers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-760
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