Direct evidence of acyl chain reversal in oxidized lipid bilayers
The oxidation of the lipids and proteins in biological membranes is implicated in a range of adverse health effects. Oxidation of unsaturated lipids can lead to the incorporation of more polar, oxygen containing groups, into the lipid tail. POPC is a common lipid in mammalian membranes. POnPC is an oxidation product of POPC containing a carbonyl group at the end of the truncated second acyl chain. It has been suggested, based on molecular dynamics simulations, that this oxidized acyl chain undergoes “acyl chain reversal” with the terminal carbonyl group orientated towards, and associating with, the head group region, rather than the tail group region of the lipid bilayer. In this proposal we plan to use neutron reflection to study the structure of POPC bilayers with increasing amounts of POnPC present to determine directly if this structural re-organization is occurring. The results will be of significance in understanding changes to the biophysical properties of cell membranes following oxidative damage.
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THOMPSON Katherine; GUSTAFSSON Emil; MICCIULLA Samantha and RENNIE Adrian R.. (2018). Direct evidence of acyl chain reversal in oxidized lipid bilayers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-763
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