Measuring the interbilayer pressure in glucolipids lamellar phases using a humidity chamber
In a recent set of experiments, we have measured the variation of the interbilayer distance with temperature on a lamellar phase composed by a natural glucolipid. At room temperature, the interbilayer distance is about 18 nm, but below -20°C, ice forms and interbilayer distances between 4.7 and 4.2 nm are measured, where the bilayer thickness is about 3 nm. In order to associate these values to the pressure exerted by ice during freezing and that for cryo-preservation applications, we want to measure the pressure corresponding to water thickness values ranging from 0.1 nm to 1.5 nm using a humidity chamber, because unfortunately the freezing device commonly used required acquisition times that are not compatible with neutron scattering. The sample is constituted by a glucolipid lamellar phase which has been largely characterized in previous SAXS experiments and on which we have collected temperature-dependent diffraction data. At the bottomlime, we plan to draw a pressure-interbilayer distance map which can be used to estimate the pressure below the water freezing point, using the interbilayer distance as common standard.
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BACCILE Niki; CRISTIGLIO Viviana; DEME Bruno and FERNANDES Francisco. (2018). Measuring the interbilayer pressure in glucolipids lamellar phases using a humidity chamber. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-783
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