Hydrocarbon Chain-Mediated Transleaflet Coupling in Asymmetric Lipid Vesicles
We propose to study the influence of hydrocarbon chain composition on transleaflet coupling in large unilamellar vesicles (size ~ 100 nm) with an asymmetric distribution of phospholipids. The asymmetric unilammelar vesicles (aLUVs) will be differently contrasted by using different combinations of deuterated phosphatidylcholines and D2O/H2O solvent. In particular we propose SANS experiments on combinations of di16:0 PC_d71 (DPPC_d71) with mixed chain lipids of increasing difference in chain length, i.e., 18:0-18:1 PC (SOPC), 16:0-18:1 PC (POPC), 16:0-14:0 PC (PMPC), 14:0-18:0 PC (MSPC) and 18:0-14:0 PC (SMPC). Data jointly analyzed with SAXS data to obtain leaflet specific structural information. This will allow us to interrogate whether or not the physicochemical state of one leaflet influences (couples to) the properties of the other.
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PABST Georg; FREWEIN Moritz Paul Karl; GERELLI YURI; MARX Lisa; PORCAR Lionel; Haden Scott and SEMERARO Enrico Federico. (2019). Hydrocarbon Chain-Mediated Transleaflet Coupling in Asymmetric Lipid Vesicles. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-822
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