The molecular organization of long-periodicity phases in the skin lipid barrier models
The extracellular lipids of stratum corneum (SC), which are organized in a lamellar pattern, prevent the dehydration of terrestrial mammals and hamper the penetration of exogenous substances through the skin. These lipids contain mainly ceramides, free fatty acids and cholesterol (Chol) mixed in an equimolar ratio. The repeating unit with the d = 13 nm is a physiologically important feature of the skin barrier, but its molecular arrangement is still under debate. An impaired skin barrier function and aberrant skin lipid organization occur in skin diseases, e.g. in recessive X-linked ichthyosis (RXLI). We prepared the simplified lipid model of RXLI with the increased fraction of skin Chol precursor - cholesteryl sulfate (CholS) at the expense of Chol. This model showed unusual polymorphic behavior modulated by the CholS content and sample annealing method. The sample with high CholS content can form a lamellar phase with the d = 10.6 nm similar to the d found in SC. The aim of the proposed study is to investigate the molecular arrangement of the lamellar phase modulated by CholS by neutron diffraction. This phase will be compared with the model of long repeat unit found in the skin.
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PULLMANNOVA Petra; DEME Bruno; ERMAKOVA Elena; KOVACIK Andrej; KUCERKA Norbert; OPALKA Lukas; SOMMEROVA Veronika and VAVROVA Katerina. (2019). The molecular organization of long-periodicity phases in the skin lipid barrier models. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-825
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