Effect of ceramide loss on the lipid ordering within the long periodicity phase of the stratum corneum
The lipid matrix of the stratum corneum (SC) in the skin contains cholesterol (CHOL), free fatty acids (FFAs) and ceramides (CERs), arranged as lamellar phases with d-spacings of ca. 5.4 nm (SPP) and ca. 12.5 nm (LPP). The LPP structure is unique to the SC and is critical to its barrier function. In skin diseases like atopic dermatitis and psoriasis, as well as in aged skin, the barrier function of the SC is compromised, and this is often associated with a change in the proportions of CER and FFA. In the studies proposed here (which build on our previous studies of healthy skin LPP models using D16), we aim to move to diseased skin LPP model systems (involving two CER, CHOL and C24 FFA) to determine how changes in the CER:FFA ratio impact on the structure of the LPP and thereby modify the skins barrier function. More specifically, we seek to determine the relative positions of the ceramide h/c chains and so determine the position and conformation of the CER and FFAs in the model systems used to mimic healthy and diseased SC. The improved understanding so gained of diseased skin will pave the way for improved treatment of these conditions.
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BARLOW David J.; AHMADI Delaram; BEDDOES Charlotte; BOUWSTRA Johanna Aaltje; DEME Bruno; FOGLIA Fabrizia; g.s.gooris; M Jayne Lawrence and NADABAN Andreea. (2019). Effect of ceramide loss on the lipid ordering within the long periodicity phase of the stratum corneum. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-826
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