Electrochemically Induced Lipid Vesicle Fusion at the Liquid-Liquid interface
The aim of this experiment is to investigate the structure and composition of floating lipid bilayers formed by vesicle fusion at the interface between two immiscible electrolyte solutions (ITIES). The approach is based on the electrostatic interaction between a previously adsorbed cationic lipid monolayer at the water/1,2-dichlorobenzene (DCB) interface with oppositely charged lipid vesicles, injected in the aqueous phase. Recent neutron reflectometry (NR) experiments performed on FIGARO at the ILL by Fragneto and co-workers have shown that such approach to form floating lipid bilayers at the water/perfluorooctane and water/d26-dodecane is promising although bilayer coverage and presence of not fused vesicles at the interface may become an issue. Finally, the vesicle fusion into lipid bilayers will be triggered by applying an electrostatic field to the ITIES.
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TUMMINO Andrea; DRYFE Robert; FRAGNETO Giovanna; GUTFREUND Philipp; Armando Maestro; Ernesto Scoppola and ZARBAKHSH Ali. (2019). Electrochemically Induced Lipid Vesicle Fusion at the Liquid-Liquid interface. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-836
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