Investigating how bioengineered monoclonal antibodies adsorb at the stainless steel water interface using neutron reflection
Monoclonal antibodies are being used to develop cutting edge drugs to fight cancers and diseases. During fill-finish manufacturing of monoclonal antibody biopharmaceuticals interactions between the antibodies and stainless steel occur (in the form of storage containers and pipelines). These interactions can lead to negative phenomena including denaturing and aggregation which, in turn, lead to a reduction of bioactivity. MedImmune has provided a bioengineered monoclonal antibody (COE-3) and we aim to investigate its adsorption dynamics (using FIGAROs high flux). As well as this the adsorbed conformation and structure of COE-3 resulting from different solution conditions can be inferred. Our group have published papers investigating the adsorption dynamics of COE-3 at the SiO_2 interface. This study will extend on this revealing how different substrates impact the adsorption of COE-3. Understanding COE-3 adsorption at the stainless steel interface will increase the efficiency of biologicals production and potentially enable to production of some, otherwise unstable, biotherapeutics.
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Jian Ren Lu; GERELLI YURI; HOLLOWELL Peter; LI Zongyi and Mingrui Liao. (2019). Investigating how bioengineered monoclonal antibodies adsorb at the stainless steel water interface using neutron reflection. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-844
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