DOI > 10.5291/ILL-DATA.9-13-851

This proposal is publicly available since 07/22/2024

Title

Cushioned lipid bilayers: spontaneous formation of intrinsically disordered protein cushions

Abstract

Solid-supported lipid bilayers are widely used for biological and fundamental studies. Since many years, researchers have been working on strategies to decouple the bilayer from the substrate in order to i) reduce the substrate effect on lipid mobility and ii) promote the formation of a hydrated region between the substrate and the bilayer. In particular, the presence of a water gap will allow permeability studies and insertion of transmembrane proteins (that would otherwise denaturate in contact with the solid). We recently observed the spontaneous formation of a protein cushion upon incubation of partially charged lipid bilayers with an intrinsically disordered protein, Histatin 5. Bilayers composed by POPC and POPS resulted to be lifted from the substrate without an loss of structural integrity. We propose now to elucidate the structural properties of this cushion by using fully deuterated phospholipids. This will allow to quantify the amount of protein present in the cushion and to estimate if any protein remains trapped within the bilayer region.

Experimental Report

Download Data

Please note that you will need to login with your ILL credentials to download the data.

Download Data

Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

Marie Skepö; Sandeep Shantaram Chakane; ERIKSSON SKOG Amanda; GERELLI YURI and JEPHTHAH Stephanie. (2019). Cushioned lipid bilayers: spontaneous formation of intrinsically disordered protein cushions. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-851

Cited by

This data has not been cited by any articles.

Metadata

Experiment Parameters

Sample Parameters

  • Formula

    • synthetic lipids
    • Histatin 5