Cushioned lipid bilayers: spontaneous formation of intrinsically disordered protein cushions
Solid-supported lipid bilayers are widely used for biological and fundamental studies. Since many years, researchers have been working on strategies to decouple the bilayer from the substrate in order to i) reduce the substrate effect on lipid mobility and ii) promote the formation of a hydrated region between the substrate and the bilayer. In particular, the presence of a water gap will allow permeability studies and insertion of transmembrane proteins (that would otherwise denaturate in contact with the solid). We recently observed the spontaneous formation of a protein cushion upon incubation of partially charged lipid bilayers with an intrinsically disordered protein, Histatin 5. Bilayers composed by POPC and POPS resulted to be lifted from the substrate without an loss of structural integrity. We propose now to elucidate the structural properties of this cushion by using fully deuterated phospholipids. This will allow to quantify the amount of protein present in the cushion and to estimate if any protein remains trapped within the bilayer region.
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Marie Skepö; Sandeep Shantaram Chakane; ERIKSSON SKOG Amanda; GERELLI YURI and JEPHTHAH Stephanie. (2019). Cushioned lipid bilayers: spontaneous formation of intrinsically disordered protein cushions. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-851