The influence of pressure on the slowing down of the short-time self-diffusion of a globular protein in the presence of multivalent ions
Within the proposed experiment, we want to investigate the effect of pressure on the slowing down of the short-time self-diffusion of a globular protein (bovine serum albumin, BSA) in the presence of multivalent ions (yttrium chloride, YCl3). The amount of bound yttrium at the protein surface can be controlled by varying the concentration of multivalent ions, resulting in a varying protein interaction strength. Here pressure shows a promising tool to control protein-interaction, because the yttrium binding is driven by dehydration. We propose to measure a sample with a fixed protein concentration with and without multivalent ions. This will help to understand the pressure effect on the hydrational properties of the yttrium binding. The variation of the protein self-diffusion under pressure will be connected to general properties of the aggregation process.
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The recommended format for citing this dataset in a research publication is in the following format:
WOLF Marcell; BECK Christian; MATSARSKAIA Olga; PETERS Judith; ROOSEN RUNGE Felix and SEYDEL Tilo. (2020). The influence of pressure on the slowing down of the short-time self-diffusion of a globular protein in the presence of multivalent ions. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-876
This data is not yet public
This data is not yet public
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