A complex model of skin lipid barrier from isolated human stratum corneum lipids
The stratum corneum (SC) prevents the dehydration of terrestrial mammals and hampers the entry of possibly harmful substances through the skin. The SC barrier lipids are cholesterol (Chol), free fatty acids (FFA), and ceramides (Cer). They are organized in a repeating sequence of broad/narrow/broad electron-lucent bands bordered by electron-dense segments with a repeat distance d = 13 nm. Skin diseases are linked with the permeability barrier dysfunction and an aberrant lipid organization. The aim of the proposal is to reconstruct the extracellular domains of SC using isolated purified human skin lipids and to determine its molecular arrangement. The individual lipid classes (or groups of the classes) will be separated by column chromatography and selectively replaced by protonated or deuterated synthetic lipids. The molecular organization of the complex model will be compared with the previously measured simple SC model based on 2 synthetic Cer. The characterized complex model can serve for further studies of the interaction of pathophysiological or therapeutic molecules with SC lipid matrix.
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The recommended format for citing this dataset in a research publication is in the following format:
PULLMANNOVA Petra; DEME Bruno; ERMAKOVA Elena; HAVRISAK Tomas; KOVACIK Andrej; KUCERKA Norbert; OPALKA Lukas; SOMMEROVA Veronika and VAVROVA Katerina. (2020). A complex model of skin lipid barrier from isolated human stratum corneum lipids. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-902
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This data is not yet public
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