Effect of cations on bacterial lipopolysaccharides (LPS) self-assembled structures
Lipopolysaccharides (LPS) is a key structural component of the outer membrane of gram-negative bacteria. LPS can trigger lethal septic shocks when released into a host’s bloodstream (i.e. the endotoxic effect). Cations can complex with LPS and lead to LPS aggregates structure transformation, which affects bacterial membrane integrity and LPS virulence. However, the effects of different cations on LPS aggregate structures are not well understood. Here, we propose a small-angle neutron scattering study (SANS) of self-assembled structures formed by different LPS chemotypes in solutions of different cations at near physiological conditions. We will vary the cation valency (comparing monovalent (Na+), divalent (Ca2+) and multivalent cations (La3+)), as well as the LPS architecture, i.e. its carbohydrate head group length (LPS-smooth, LPS-Ra and LPS-Rd). We also propose to investigate the temperature effect on these systems, as it has been shown in preliminary studies to have a profound effect on the self-assembled structure.
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BRISCOE WUGE H; BHARATIYA Bhaveshkumar; GUO Xueying; Charlotte Kenton; Lauren Matthews; SCHWEINS Ralf; SLASTANOVA Anna; STEVENS Michael and WANG Gang. (2020). Effect of cations on bacterial lipopolysaccharides (LPS) self-assembled structures. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-911
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