Diffusive dynamics in crowded solutions containing two types of proteins
We have successfully investigated "binary" mixtures of proteins, namely bovine serum albumin (BSA) and polyclonal bovine gamma-globulin (Ig) in aqueous (D2O) solutions using IN16B in its high-resolution setup employing the Doppler drive. The center-of-mass diffusion of the proteins observed in these mixtures can be understood in terms of the total volume fraction of the mixture. We wish to add data using BATS (1) to access lower total volume fractions corresponding to overall faster motions better probed by the wider energy range of BATS; (2) to focus on the protein internal dynamics. By global fits describing the energy and momentum transfers due to the internal dynamics simultaneously, even minute changes in the internal dynamics of the different proteins will be accessible. Previous panel comment: "Very nice proposal, improved since last submission, taking into account all comments. Should be done." It will benefit from the world-leading signal-to-noise ratio of BATS and our experience with QENS data from complex mixtures such as tracer proteins in cellular lysate [M.Grimaldo et al., J.Phys.Chem.Lett. vol.10, p.1709 (2019)], which we studied by experiment and simulations.
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COLIN Louise; APPEL Markus; BECK Christian; GRAPENTIN Christoph; MATSARSKAIA Olga; POUNOT Kevin; ROOSEN RUNGE Felix; Frank Schreiber and SEYDEL Tilo. (2021). Diffusive dynamics in crowded solutions containing two types of proteins. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-952
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