Influence of lipid-synthesizing enzymes on lipid transfer between membranes.
Non-vesicular lipid transport is an essential process to ensure membrane biogenesis and organelle function. In cells, the mechanisms involved in non-vesicular lipid transport is still poorly understood. Some lipid transfer proteins (LTPs) have been identified and the enzymes that synthesize lipids are suspected to play a role in this process. The objective of our study is to investigate whether Cho1, a yeast phosphatidylserine (PS) synthase, by itself and/or in concert with the LTP Osh6, is involved in the transfer of newly synthesized PS between membranes by using time-resolved small angle neutron scattering (TR-SANS). To do so, in vitro transport kinetics of newly synthesized hydrogenated PS (H-PS) from a deuterated (D) donor liposome to an acceptor D-liposome will be analyzed in different liposome reaction mixtures (Cho1 alone, Cho1 + Osh6 in different molecular ratios). From the decay curves, we will determine and compare essential transport parameters, like the transfer exchange constant or the half time of lipid transport for each condition. We expect to demonstrate that the active synthesis of lipid, in concert with LTPs, plays a key role in non-vesicular lipid transfer.
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MICHAUD Morgane and PORCAR Lionel. (2021). Influence of lipid-synthesizing enzymes on lipid transfer between membranes.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-980
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