Unraveling Trans-Membrane Coupling Mechanisms in PE-PC-Sphingomyelin containing Asymmetric Lipid Vesicles
We have evidence for trans-bilayer coupling mechanisms in asymmetric large unilamellar lipid vesicles, containing either combinations of phosphatidylethanolamine (PE) and phosphatidylcholine (PC), or PC and the highly chain-asymmetric milk sphingomyelin (MSM). The exact mechanisms therefore are however still unclear, as there might be contributions from H-bonding networks and dynamic chain interdigitation. Beamtime on IN15 and D22 would allow us to separate these contributions by the study of trans-membrane structure and bending fluctuations in 3 asymmetric systems: first, PC in the outer leaflet, opposed to the H-bonding PE, a system which is structurally coupled in the gel phase. Second, we use MSM instead of PC, introducing interdigitation stress by the long acyl chains in MSM. Third is an intermediate case, using a 1:1 mixture of PC and MSM in the outer leaflet. We will be thereby able to estimate the contribution strength of either mechanism.
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FREWEIN Moritz Paul Karl; CZAKKEL Orsolya; HOFFMANN Ingo; PABST Georg; PILLER Paulina and PORCAR Lionel. (2021). Unraveling Trans-Membrane Coupling Mechanisms in PE-PC-Sphingomyelin containing Asymmetric Lipid Vesicles. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-997
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