The use of magnetic reference layers to unravel interaction of proteins with complex lipid bilayers
In previous work, we have characterised the encapsulation of several industrially relevant proteins, aspartic protease, ß-galactosidase ) and sugar beet phytoglobin into lipid sponge phases. In the present study we will investigate important aspects of the encapsulation of iron binding hem-proteins, e.g. myoglobin, in the sponge phase, namely the protein lipid interaction. These type heme-bound iron are used to treat anemia instead of iron in organic salts conventionally used. Plant haemoglobins (or phytoglobins) are here specially interesting as the address one of the challenges with plant-based food, namely supply of sufficient iron that has high bioavailability. The reason for encapsulation is preventing unwanted proteolytic reaction and redox reactions. Neutron reflectometry is the only technique that can be used to characterise the interaction between the heme protein and the lipid at the interface. Here we will utilise the difference in scattering length density between a protein and hydrogenated lipid in combination with solvent contrast matching. The use of magnetic reference layers provides additional contrasts.
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The recommended format for citing this dataset in a research publication is in the following format:
NYLANDER Tommy; DEVISHVILI Anton; Jennifer Gilbert; LUCHINI Alessandra; MACHINGAUTA Marshall and Alexei Vorobiev. (2023). The use of magnetic reference layers to unravel interaction of proteins with complex lipid bilayers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.CRG-2965
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