Photoinduced oligomerization of the transcription factor EL222
Light controls the structure and assembly of photosensory proteins like cryptochromes, phytochromes and light-oxygen-voltage (LOV) proteins. For instance, EL222 is a light-activated LOV-containing transcription factor that interacts with its target DNA only when illuminated with blue light. EL222 is known to form multimers upon light irradiation but the molecular mechanism and kinetics are largely unknown. Here we propose to employ small-angle neutron scattering (SANS) to uncover the oligomeric intermediates in the photocycle of EL222. SANS will be utilized to render low-resolution structural models of the photo-oligomers as well as to gain insight into the thermodynamics and kinetics of cluster formation and dissociation. We expect that our results will pave the way for the study of light-induced protein oligomerization phenomena. Moreover, the acquired knowledge may help in the design of photo-oligomerization systems for regulating protein-protein interactions with light.
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Gustavo Fuertes; CHAUDHARI Aditya and MARTEL Anne. (2020). Photoinduced oligomerization of the transcription factor EL222. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.DIR-209
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