Use polarisation analysis to separate coherent from incoherent elastic scattering intensities with protonated and per-deuterated GFP.
There is a growing number of indications that quantum mechanics might play a role for the functioning of certain biomolecules, as for instance through tunnelling effects. As quantum effects depend on the mass of the scatterer, their signature should be detectable when comparing a protonated protein with its per-deuterated counterpart. We use both versions of GFP powders (already disposable). We aim at analyzing elastic scattering data acquired with IN5 TOF spectrometer. However it is not straightforward that the signal of our dGFP protein in D20 should be due to incoherent scattering of hydrogens, due to the lower incoherent cross-section of deuterium. We carried out a numerical analysis to estimate the coherent fraction of scattering, which gave us 50%. Experiments on IN12 yielded up to 40% of coherent scattering. Thus it is paramount to separate both contributions to analyze correctly our data. We want to do polarisation analysis on D7 at room temperature on both samples, empty cell and Vanadium. With a 4.8 A incoming wavelength, we can investigate Q from 0.3 to 2.5 A^-1, which is also our IN5 Q range. It should take 2 hours per sample and 1 for calibration.
The data is currently only available to download if you are a member of the proposal team.
The recommended format for citing this dataset in a research publication is in the following format:
NIDRICHE Agathe; MANGIN-THRO Lucile and PETERS Judith. (2021). Use polarisation analysis to separate coherent from incoherent elastic scattering intensities with protonated and per-deuterated GFP.. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.EASY-1064
This data is not yet public
This data is not yet public
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