Additional buffer condition for a SEC-SANS study of the ion channel DeCLIC
Pentameric ligand-gated ion channels play key roles in converting chemical to electrical signals in the nervous system. Bacterial homologs are valuable structure-function model systems in this complex superfamily. We previously collected SEC-SANS data for the bacterial channel DeCLIC in deuterated detergent. This gave us data from monodisperse protein with a hidden micelle, revealing subtle effects of the modulator calcium on the conformational ensemble. We recently discovered a new open state of DeCLIC, favored below pH 7. We therefore seek to complete our characterization by collecting additional SEC-SANS data at low pH. Based on previous runs (see workflow https://tinyurl.com/yndea6um ), one 5-hour session would be sufficient to collect the most critical sample and buffer reference; if another 5-hour slot were available, we would also collect another control with calcium. These results would enable comparative modeling of DeCLIC modulation by two physiologically relevant stimuli, calcium and pH; and would provide validation for our new cryo-electron microscopy structures, possibly representing unexplored regions of the gating landscape.
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LYCKSELL Marie and MARTEL Anne. (2021). Additional buffer condition for a SEC-SANS study of the ion channel DeCLIC. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.EASY-861
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