Structural characterization of supported membrane hosting Tissue Factor
We are investigating the formation of supported membranes by depositing on a solid substrate protein-loaded nanodiscs (ND). Nanodiscs are discoidal lipid bilayers surrounded by a protein belt. The present work will be based on a on a “cut-up” version of the NDs, named peptide-discs, where the nanodisc belt is composed by self-assembled peptide (named 18A) molecules. Recently, we have so far conducted a pilot study which show that the formation of a macroscopic lipid bilayer can indeed be mediated by peptide-disc deposition and subsequent removal of the peptide molecules by buffer rinsing. We are now interested in testing the method for the preparation of supported membranes hosting oriented membrane proteins. The membrane protein we propose to investigate is Tissue Factor (TF). TF is the membrane protein that initiates blood coagulation upon binding with the soluble protein activated factor VII (FVIIa). Here we propose to produce supported membranes hosting TF through deposition of TF-loaded peptide-discs and to characterize their structure by means of NR.
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The recommended format for citing this dataset in a research publication is in the following format:
LUCHINI Alessandra; Raul Araya-Secchi; ARLETH Lise; FRAGNETO Giovanna; MICCIULLA Samantha and F.G. Tidemand. (2020). Structural characterization of supported membrane hosting Tissue Factor. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-874
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