SARS CoV-2 Spike protein is remodelling HDL. What are the structural consequences of this building using different HDL lipid compositions?
SARS-CoV-2 Spike (S) protein binds cholesterol and high density lipid protein (HDL) and affects the function of HDL (incl. ability to exchange lipids). Indeed, thanks to neutron reflection experiments, we found out that both, the model donor membrane¿s composition as well as the presence of the S protein affects the HDL¿s capacity to deposit and exchange lipids with a model membrane. Moreover, the S protein takes preferably DMPC and PLPC when in presence of cholesterol and is not uptaking POPC. We now want to verify that the function of HDL is affected through the selective removal of lipids from HDL by S protein. Hence, we plan to examine the interaction of HDL with different lipid compositions with a model membrane when incubated with and without the S protein. Thereby we will use reconstructed (-r) HDL made of DMPC, PLPC or POPC and an deuterated DMPC model membrane, since we know the S protein does not removes saturated lipids such as DMPC without cholesterol at all. We expect to observe no effect of the S protein on the lipid exchange ability of r-HDL made of POPC, while a modulation of the r-HDL function should be expected for particles containing DMPC and PLPC.
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CARDENAS; Yubexi Correa; DEL GIUDICE Rita; FELDERER Birgit; GVARAMIA Manuchar; Favour Amarachi Nzekwe; Nicolò Paracini and Noelia Soto Gomez. (2023). SARS CoV-2 Spike protein is remodelling HDL. What are the structural consequences of this building using different HDL lipid compositions?. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-02-997
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