DOI > 10.5291/ILL-DATA.8-03-891

This proposal is publicly available since 11/14/2021

Title

Towards a more realistic model system for the cellular crowded environment

Abstract

Using deuterated cellular lysate obtained from living cells, we will to establish a natural crowding environment for protonated "tracer" or "target" proteins. We will systematically investigate the influence of this natural crowding environment on both the conformation and interaction of of the target proteins using SANS. We have previously tested the feasibility of this experiment, and we have been allocated the required preparation of the lysate by the ILL deuteration laboratory through a successful peer-reviewed D-lab proposal.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

BECK Christian; CZAKKEL Orsolya; Juliette M. Devos; FEUSTEL Michal; FORSYTH Trevor; GRIMALDO Marco; HAERTLEIN Michael; MOULIN Martine; ROOSEN-RUNGE Felix; Frank Schreiber; SCHWEINS Ralf; SEYDEL Tilo; SOHMEN Benedikt and ZHANG Fajun. (2016). Towards a more realistic model system for the cellular crowded environment. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-891

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Metadata

Experiment Parameters

  • Environment temperature

    290-310
  • Experiment moment

    full q-range

Sample Parameters

  • Formula

    • Protein in aqueous solution