Towards a more realistic model system for the cellular crowded environment
Using deuterated cellular lysate obtained from living cells, we will to establish a natural crowding environment for protonated "tracer" or "target" proteins. We will systematically investigate the influence of this natural crowding environment on both the conformation and interaction of of the target proteins using SANS. We have previously tested the feasibility of this experiment, and we have been allocated the required preparation of the lysate by the ILL deuteration laboratory through a successful peer-reviewed D-lab proposal.
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BECK Christian; CZAKKEL Orsolya; Juliette M. Devos; FEUSTEL Michal; FORSYTH Victor Trevor; GRIMALDO Marco; HAERTLEIN Michael; MOULIN Martine; ROOSEN RUNGE Felix; Frank Schreiber; SCHWEINS Ralf; SEYDEL Tilo; SOHMEN Benedikt and ZHANG Fajun. (2016). Towards a more realistic model system for the cellular crowded environment. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-03-891