DOI > 10.5291/ILL-DATA.8-04-759

This proposal is publicly available since 10/30/2020

Title

External crowding in protein solutions

Abstract

The interior of living cells is occupied by macromolecules such as proteins, which occur at a high volume fraction on the order of 30% in the aqueous solution of the cellular fluid. The issue of macromolecular crowding is therefore of primordial importance for the function of living cells [R.J. Ellis, Curr. Opin. Struct. Biol. 2001, 11, 114]. To model the situation of crowding in vitro, we propose to investigate the diffusion in (1) protein (BSA) - protein (IgG) - water (D2O) ternary mixtures, where the proteins BSA (bovine serum albumin) and IgG (immunoglobulin) are important constituents of blood; (2) protein(IgG)-crowding agent (deuterated PEG) - water (D2O) ternary mixtures. Using our previous large QENS data sets of BSA-water and IgG-water binary mixtures and consistent modeling, we expect to be able to separate the different contributions to the signal in the QENS spectra in the ternary mixture. Moreover, we have comprehensively characterized the involved samples using complementary techniques such as SAXS/SANS and SLS/DLS.

Experimental Report

Download Data

Please note that you will need to login with your ILL credentials to download the data.

Download Data

Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

GRIMALDO Marco; BECK Christian; DA VELA Stefano; FEUSTEL Michal; ROOSEN-RUNGE Felix; Frank Schreiber; SEYDEL Tilo; SOHMEN Benedikt and ZHANG Fajun. (2015). External crowding in protein solutions. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-759

Cited by

This data has not been cited by any articles.

Metadata

Experiment Parameters

  • Environment temperature

    280 - 370K
  • Experiment energy

    6.27A
  • Experiment moment

    0.5 - 1.9/A
  • Experiment res energy

    0.9 micro eV FWHM

Sample Parameters

  • Formula

    • Bovine Gamma-Globulin (IgG), Bovine Serum Albumin (BSA), d-(poly)ethylene glycol, D2O