DOI > 10.5291/ILL-DATA.8-04-761

Please note

The full details of this dataset is not yet available to the public as it still under its embargo period. As such there are only a few details publically exposed. To find out more about how the ILL governs the release of data, please go here. Thank you for your understanding.

Title

Dynamics of a whole family of cyanfluorescent proteins explored by neutronscattering

Abstract

Cyan Fluorescent Proteins (CFPs) are widely used in FRET-based live cell imaging experiments, in which they non-radiatively transfer their excitation energy to a Yellow Fluorescent Protein depending on their vicinity, thus providing a technique to probe protein-protein interactions. The prototypal CFP, Enhanced Cyan Fluorescent Protein (ECFP), suffers from non-optimal fluorescence properties that were not drastically improved in two later-developed CFPs, Cerulean and SCFP3A. Two additional rounds in optimization by structure- based mutagenesis yielded mTurquoise and mTurquoise2, the latter being the monomeric fluorescent protein with the highest fluorescence efficiency (fluorescence quantum yield). A structural analysis by X-ray crystallography revealed a flexibility of one of the beta-strand of the protein, whose interactions with the chromophore could affect its fluorescence properties. In the latest CFPs, this flexibility appeared to have been suppressed. By using neutron scattering, we would like to bring a definitive experimental proof of the mechanism of fluorescence-controlled of the chromophore by its protein matrix by completing the previous experiment.

Experimental Report

Download Data

The data is currently only available to download if you are a member of the proposal team.

Download Data

Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

ROYANT Antoine; CLAVEL Damien; GOTTHARD Guillaume; GUILLON Virginia; LAFAYE Celine; MARTINEZ Nicolas; PETERS Judith and VON STETTEN David. (2015). Dynamics of a whole family of cyanfluorescent proteins explored by neutronscattering. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-761

Cited by

This data has not been cited by any articles.

Metadata

Experiment Parameters

This data is not yet public

Sample Parameters

This data is not yet public