DOI > 10.5291/ILL-DATA.8-04-761

This proposal is publicly available since 07/02/2020


Dynamics of a whole family of cyanfluorescent proteins explored by neutronscattering


Cyan Fluorescent Proteins (CFPs) are widely used in FRET-based live cell imaging experiments, in which they non-radiatively transfer their excitation energy to a Yellow Fluorescent Protein depending on their vicinity, thus providing a technique to probe protein-protein interactions. The prototypal CFP, Enhanced Cyan Fluorescent Protein (ECFP), suffers from non-optimal fluorescence properties that were not drastically improved in two later-developed CFPs, Cerulean and SCFP3A. Two additional rounds in optimization by structure- based mutagenesis yielded mTurquoise and mTurquoise2, the latter being the monomeric fluorescent protein with the highest fluorescence efficiency (fluorescence quantum yield). A structural analysis by X-ray crystallography revealed a flexibility of one of the beta-strand of the protein, whose interactions with the chromophore could affect its fluorescence properties. In the latest CFPs, this flexibility appeared to have been suppressed. By using neutron scattering, we would like to bring a definitive experimental proof of the mechanism of fluorescence-controlled of the chromophore by its protein matrix by completing the previous experiment.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

ROYANT Antoine; CLAVEL Damien; GOTTHARD Guillaume; GUILLON Virginia; LAFAYE Celine; MARTINEZ Nicolas; PETERS Judith and VON STETTEN David. (2015). Dynamics of a whole family of cyanfluorescent proteins explored by neutronscattering. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-761

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Experiment Parameters

  • Environment temperature

    20 - 315 K
  • Experiment energy

    2.2 A, 6.2 A and 5.1

Sample Parameters

  • Formula

    • Cerulean
    • ECFP
    • mTurquoise2