Towards a realistic model of the cellular environment: Dynamics in 'naturally' crowded protein solutions
Using deuterated cellular lysate obtained from living cells, we will establish a natural crowding environment for protonated "tracer" or "target" proteins. We will systematically investigate the influence of this natural crowding environment on both the global center-of-mass diffusion as well as the internal diffusive motions of the target proteins using IN16B. We have previously tested the feasibility of this experiment, and we have been allocated the required preparation of the lysate by the ILL deuteration laboratory through a successful peer-reviewed D-lab proposal. We will analyze the data using the frameworks successfully established by our group [cf. M.Grimaldo et al., EPJ Web of Conferences 83, 02005 (2015) and references therein] .
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BECK Christian; Juliette M. Devos; FEUSTEL Michal; FORSYTH Trevor; GRIMALDO Marco; HAERTLEIN Michael; MOULIN Martine; ROOSEN-RUNGE Felix; Frank Schreiber; SEYDEL Tilo; SOHMEN Benedikt and ZHANG Fajun. (2016). Towards a realistic model of the cellular environment: Dynamics in 'naturally' crowded protein solutions. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-774