DOI > 10.5291/ILL-DATA.8-04-785

This proposal is publicly available since 10/03/2021

Title

Intracellular and transmembrane water dynamics of Shewanella oneidensis bacteria at 500 and 1500 MPa

Abstract

We propose to measure intracellular and transmembrane water dynamics in microbes at extremely high pressure. We will study S. oneidenisis that provides a model for extremophile behaviour and that we have previously adapted to survive into the GigaPascal pressure range. QENS experiments on H- vs D-labelled cells suspended in H/D media using specially developed HP devices at IN5 or IN6 will allow us to observe intracellular water mobility under extremely high pressure for the first time, and examine pressure effects on water transport through transmembrane Aquaporin channels. The results will give a unique view on microbial functioning under extreme conditions.

Experimental Report

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Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

MCMILLAN Paul; BOVE Livia Eleonora; FOGLIA Fabrizia; Rachael Hazael; KOZA Michael Marek; MEERSMAN Filip; OLLIVIER Jacques; WILDING Martin and ZBIRI Mohamed. (2016). Intracellular and transmembrane water dynamics of Shewanella oneidensis bacteria at 500 and 1500 MPa. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.8-04-785

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Metadata

Experiment Parameters

  • Environment temperature

    ambient
  • Experiment energy

    lambda 5 Ang
  • Experiment res energy

    70 ueV

Sample Parameters

  • Formula

    • H/D substituted Shewanella oneidensis bacteria (no known physiological/medical hazard) suspended in PBS (phosphate/saline) aqueous buffer solution