Solubilising protein nanoconstructs in deep eutectic solvents with tailored hydrophobicity
Deep eutectic solvents (DESs) have emerged as promising solvents for the solubilization and preservation of biomolecules, in particular proteins. DESs are obtained through the complexation of simple organic molecules, where the physicochemical properties of the solvent can be tailored through different combinations of precursors. Although their tailorable character expands the potential range of bio-related applications of DESs, we found that some DESs lead to rapid protein denaturation or show very low protein solubility. To overcome these, we began an investigation on the behaviour of protein-polymer-surfactant nanoconstructs in DESs. These nanoconstructs allow stabilising proteins in different DESs with increased stability compared to aqueous buffers and wild-type proteins. Here, we will investigate the conformation of two protein nanoconstructs, modified myoglobin and lipase, in DESs with a wide range of properties, from hydrophilic to hydrophobic. The results from this investigation will be used to elaborate a framework to design biobased systems using DES, where the properties of the solvent can be optimised for new applications.
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Adrian Sanchez-Fernandez; Alex P. S. Brogan; Susana M. Meza H.; NICHOLSON Jake and PREVOST Sylvain. (2023). Solubilising protein nanoconstructs in deep eutectic solvents with tailored hydrophobicity. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1062
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