Why do we sigh?: Simultaneous structural and mechanical characterization of model pulmonary surfactant interfaces
The human lung¿s internal surface is coated by a lipidic lung surfactant (LS) interface, whose dysfunction leads to pulmonary collapse. To operate properly, LSs must respond to dilatational and compressional deformations imposed by breathing, dynamically adjusting their composition and structure through pulmonary proteins located within membrane reservoirs buried below the interface. Although LS morphology has been investigated in quiescent conditions, no studies have interrogated this aspect under physiological breathing, particularly during involuntary sighs, which are crucial to LS conditioning. We propose to interrogate LS interfaces via neutron reflectometry, to elucidate the changes in composition and thickness of the interface and associated reservoirs during dynamic conditions mimicking inhalation, exhalation, and sighing. We will perform experiments on a custom Quadrotrough, which can apply controlled compressional and dilatational deformations at physiological temperatures, strains, and frequencies. These experiments will correlate morphological information with the interface¿s mechanical properties, revealing the dynamic structural hallmarks of pulmonary physiology.
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Mariana Rodriguez-Hakim; Collada, A; GUTFREUND Philipp; NOVAES SILVA Maria Clara; PEREZ-GIL Jesus; Kiet Pham; Miguel A. Rubio; P. Sánchez-Puga; TAJUELO RODRIGUEZ Javier; THOMPSON Benjamin; VERMANT Jan and WAGNER Norman. (2024). Why do we sigh?: Simultaneous structural and mechanical characterization of model pulmonary surfactant interfaces. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-1109
This data is not yet public
This data is not yet public
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