DOI > 10.5291/ILL-DATA.9-13-620

This proposal is publicly available since 12/09/2020

Title

Kinetics of two-step nucleation in protein crystallization studied by real-time SANS

Abstract

In this proposal we aim to study the two-step nucleation mechanism in protein crystallization by real-time SANS. With the larger volume and no radiation damage of SANS, we can extend our previous successful study using SAXS (A. Sauter et al., 2015, J. Am. Chem. Soc., 137, 1485) to a broad experimental conditions. This is extremely important for establishing the real-time method and providing systematic experimental results for our understanding of the nonclassical pathways of protein crystallization.

Experimental Report

Download Data

Please note that you will need to login with your ILL credentials to download the data.

Download Data

Data Citation

The recommended format for citing this dataset in a research publication is in the following format:

ZHANG Fajun; DA VELA Stefano; FEUSTEL Michal; GRIMALDO Marco; MATSARSKAIA Olga; ROOSEN-RUNGE Felix; Frank Schreiber; SCHWEINS Ralf; SEYDEL Tilo and SOHMEN Benedikt. (2015). Kinetics of two-step nucleation in protein crystallization studied by real-time SANS. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-620

Cited by

This data has not been cited by any articles.

Metadata

Experiment Parameters

  • Environment temperature

    293K
  • Experiment energy

    6 A
  • Experiment moment

    0.007 - 0.35 A^-1

Sample Parameters

  • Formula

    • BLG with YCl3
    • BLG with ZnCl2
    • BLG with CdCl2