Characterising supported lipid bilayers from selectively deuterated PC and cholesterol, and their interactions with human HDL
Atherosclerosis is the leading cause of death in western society, its consequences of cardiovascular diseases such as strokes and heart attacks arise from lipoprotein deposition onto cell membranes in the artery walls. Essential information regarding molecular mechanisms resulting in plaque build-up, is missing. Neutron reflection with selective deuteration studies provide crucial and unique information about lipid exchange and lipoprotein binding to model cell membrane. We have so far established a successful protocol to follow such exchange processes based on perdeuterated lipids. However, the availability of deuterated lipid types and molecular species is quite limited. Thanks to a collaboration with the Life Sciences group at the ILL using the D-Lab, a recently started ILL PhD studentship and chromatographic protocols already established at our home institution, full extracts of D2O-contrast matched phosphatidylcholine (PC) and POPC as well as their mixtures with cholesterol will be used to form supported lipid bilayers and follow the interactions with high density lipoprotein (HDL). This will unravel the effect of lipid composition of model cell membranes and HDL interactions.
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CARDENAS; Kathryn Browning; FORSYTH Victor Trevor; HAERTLEIN Michael; LONE Abdullah; Armando Maestro; MARIC Selma; MOULIN Martine; NIELSEN Josefine Eilsoe; Sarah Waldie and Florian Weber. (2017). Characterising supported lipid bilayers from selectively deuterated PC and cholesterol, and their interactions with human HDL. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-681
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