Antimicrobial peptide loaded nanogels and their interaction with lipid bilayers
Due to increasing antibiotic resistance, antimicrobial peptides (AMPs) are of increasing interest as alternatives to classical antibiotics. Usually, AMPs are bactericidal via disruption of the bacterial membrane. However, AMPs tend to be degraded by the chemical and enzymatic environment in vivo, it is therefore important to protect the AMPs in drug delivery systems (DDS) to ensure effect after administration. Nanogels, designed to be biocompatible, provide a water-rich environment where the AMP can maintain its function, giving a suitable DDS for AMPs. The mechanisms of action of DDSs are important to map. Ellipsometry data indicates an interesting synergistic effect of our proposed nanogel and AMP in the formulation. Initial reflectometry data shows that it is possible to see a difference between a bacteria mimicking PC:PG bilayer before and after introduction of AMP. Employing further contrasts should be able to elucidate whether the peptide is located in the bilayer or is also removed upon washing. We aim to compare these results to AMP-loaded nanogels to measure if the disruption mechanism changes upon encapsulation.
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MALMSTEN Martin; Kathryn Browning; Liv Sofia Elinor Damgaard; Armando Maestro; NORDSTROM Randi and Elisa Parra-Ortiz. (2018). Antimicrobial peptide loaded nanogels and their interaction with lipid bilayers. Institut Laue-Langevin (ILL) doi:10.5291/ILL-DATA.9-13-733
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